WebApr 10, 2024 · However, comparative analyses of a recently established Runx2-conditional knockout mouse line (Runx2-flox) with Runx1 - and Runx3-flox lines showed that Runx2 plays a smaller role as a tumor-promoting factor in p53-deficient OS and T-cell lymphoma than do Runx3 and Runx1, respectively [32,53]. We then tested the functionality and reversibility of our system for conditional knockdown of endogenous TP53 gene expression in vitro and in vivo. By subjecting human breast cancer MCF7 cells transduced with a Tet-on TP53-targeted shRNA vector to doxycycline cycling, we demonstrated efficient and … See more We took advantage of the promiscuous repression activity of tTRKRAB, a fusion protein between the Krüppel-associated box (KRAB) domain … See more Next, we tested the performance of Tet-on and Tet-off versions of the system in vivo. Expression of glial cell line-derived neurotrophic factor … See more Finally, we analyzed the performance of both versions of the conditional system in transgenic mice generated by perivitelline injection of fertilized oocytes with the lentiviral vectors30. … See more Next, based on our previous demonstration that Pol III promoter−driven shRNA expression cassettes can be efficiently regulated … See more
p53 knockout mice (–/–) are more susceptible than (+/–) or (+/+) …
WebNov 22, 2024 · Braf V600E-induced prostate carcinoma in conditional p53-deficient mice. ... CD24, CD133, or CD326 knockdown modulates KrasG12D/p53 L/L PCA cell growth, motility, invasion, and stemness. WebComparing the transcriptomes of oncogenic Kras cells and Kras knockout cells, we identified 603 genes that were specifically up-regulated in Kras knockout cells, including the Fas gene, which encodes a cell surface … law passed in mississippi
Conditional RNA interference in vivo to study mutant p53 …
WebMar 11, 2008 · In the Nf2F; p53F mice, tumors ranged from nodular, noninvasive visceral to nodular, locally invasive visceral and invasive parietal growth patterns. In the sarcomatoid tumors invading muscle layers, a typical “signet-cell”-like picture was observed, especially when cells invaded the esophagous. WebJan 28, 2013 · Knockdown of mutant p53 inhibits the growth and viability of 5637 and T24 cells We next investigated the effects of knockdown of mutant p53 on bladder cancer … WebJan 25, 2024 · using p53 conditional knockout mouse in esophageal epi-thelium. is precancerous lesions model is well suited for. exploring the mechanism of esophageal cancer and finding a. lavrov russia ukraine