Myotube fusion index

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August undefined, 2024

WebMar 31, 2024 · The final ATAC and gene expression libraries were sequenced on an Illumina Novaseq 6000 with v1.5 Kit. For the ATAC libraries, 24 bp of cell barcode and spacer, 8 bp single index read, and 100 bp paired-end reads were generated; for the gene expression libraries, 100 bp paired-end reads and 10 + 10 index reads were generated. WebMyotube Formation. In fact, myotube formation resulted significantly affected by both PANi concentration and nanofiber alignment according to the myosin heavy chain (MHC) …

ViaFuse: Fiji macros to calculate skeletal muscle cell …

WebJun 22, 2024 · The fusion index was determined to estimate the fusion capacity of the cultured myoblasts within the different stimulation conditions. The fusion index was found to be slightly increased in the 22 h per day stimulation condition (77 ± 7%) compared to statically cultured myotubes (69 ± 9%) ( Figures 2A,B ). WebNov 4, 2024 · The fusion index denotes the ratio of the nuclei number in myocytes with multiple nuclei, and the maturation index is the proportion of myotubes having five or … tall kitchen units for sale

Fine-Tuning of Piezo1 Expression and Activity Ensures Efficient

WebJun 22, 2024 · The fusion index was determined to estimate the fusion capacity of the cultured myoblasts within the different stimulation conditions. The fusion index was … WebMyotubes and fusion index quantification? Hi, I am brand new in the muscle field and I was wondering what is the best (and fast) magnification to quantify myotubes and fusion index commonly... WebJan 24, 2024 · Our study shows that Piezo1 is essential for terminal muscle differentiation acting on myoblast fusion, suggesting that Piezo1 deregulation may have implications in muscle aging and degenerative diseases, including muscular dystrophies and neuromuscular disorders. tall kitchen table with 2 chairs

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TGFβ signaling curbs cell fusion and muscle regeneration

WebMyoblast fusion is essential for formations of myofibers, the basic cellular and functional units of skeletal muscles. Recent genetic studies in mice identified two long-sought … WebMar 16, 2024 · Myoblast fusion, which is essential for muscle development, regeneration, and repair, can be assessed in vitro via the calculation of a fusion index. Traditionally, this … two shades cafeWebApr 19, 2024 · High concentrations of arachidonic acid have a deleterious effect on both myoblast proliferation, myotube area, and myotube fusion. Our data demonstrates that arachidonic acid reduced proliferation by 85% and 32%, myotube area by 63% and 38% and myotube fusion index by 93% and 50% respectively (P<0.001 for all). tall kitchen unit carcass

"WebJun 22, 2024 · In 2D, enhanced myogenesis was observed, as evidenced by an increased myotube diameter and fusion index. Second, 2D results were translated towards 3D … " - Myotube fusion index

Myotube fusion index

Does anyone has a protocol for the calculation of C2C12 …

WebTherefore, to examine the degree of differentiation, we compared the number of nuclei within myotubes (fusion index) and the myotube width cultured on each surface. C2C12 myotubes and nuclei on day 5 of differentiation were fluorescently visualized with α-actinin and DAPI, respectively, and the number of nuclei in the α-actinin-positive area ... Web例文 Myotube formation was quantified by differentiation index, fusion index, and myonuclei number per myotube (see below for detailed description). 筋管形成は、分化指数、融合指数、および筋管あたりの筋核数によって定量化されました（詳細な説明については以下を参 …

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WebApr 26, 2024 · Myotube fusion index was evaluated and defined as the ratio of myonuclei on myotubes containing more than two nuclei. The results are expressed as the mean ± SEM of three independent cultures. Significant differences from the means of control siRNA cultures (black bar) at p < 0.05 or p < 0.01 are indicated by single or double asterisks. WebTo analyse fusion index, the nuclei of 60–90 myotubes in each well were counted to obtain the average number of nuclei per myotube. Immunostaining For immunofluorescent detection of different phenotypes of muscle satellite cells under different conditions, the following antibodies were used: rabbit polyclonal anti-MyoD (1:100, Santa Cruz ...

WebJun 28, 2024 · Myotube diameter and nuclear fusion index (NFI). The effect of ex vivo human serum-conditioned media on myotube diameter and NFI in vitro. A: representative images illustrating atrophy in myotubes conditioned with serum from older males in comparison to untreated control C2C12 myotubes and myotubes treated with serum from … WebThe fusion index was calculated as the ratio of the nuclei number in myocytes with two or more nuclei versus the total number of nuclei. (A) Hybrid patterns with different arc …

WebSep 3, 2024 · The myotubes fusion rate was assessed by counting the number of nuclei in differentiated myotubes (>2myonuclei) as a percentage of the total number (mononucleated). Cell scratch assays WebApr 11, 2024 · In contrast to the proliferation behavior indicated in Fig. 1d, the experimental groups (G2 and G3 with low FBS content) exhibit a high fusion index of 55.6% and 58.8%, respectively, while the ...

WebNov 4, 2024 · The fusion index denotes the ratio of the nuclei number in myocytes with multiple nuclei, and the maturation index is the proportion of myotubes having five or more nuclei in a single myotube.

WebMyotubules are multinucleated cells which occur from cells fusing together and are the precursor to muscle fibers. The fusion index is calculated by staining the fixed cell and … two shades of blue karaokeWebApr 29, 2024 · Increased myoblast regenerative capacity was observed as shown by the increased number of nuclei per myotube, fusion index, and maturation index. Conclusions: Ginger extracts exerted their potency in promoting muscle regeneration as indicated by prevention of cellular senescence and promotion of myoblast regenerative capacity. two shades cafe menuWebDec 16, 2024 · This results in multiple close myoblasts being counted as one myotube which further inflates the fusion index. MyoCount can count MYH-positive cells with less than three nuclei in them to generate fusion index automatically; however, this number is not fully accurate because of the aforementioned issues with resolving nuclear clumps, counting ... two shades of blues film 1999Webnoun. myo· tube ˈmī-ə-ˌt (y)üb. : a developmental stage of a muscle fiber composed of a syncytium formed by fusion of myoblasts. two shades of blue full movieWebMyotube fusion index was determined by counting the nuclei in every myotube (defined as MyHC‐positive cells containing ≥2 nuclei) per field and dividing by the total number of nuclei in the field. Images were analyzed using ImageJ, which was downloaded from the National Institutes of Health website (Bethesda, MD, USA) [ 15 ]. tall kitchen trash cansWebNational Center for Biotechnology Information tall kitchen units for ovensWebNational Center for Biotechnology Information two shades of blue lyrics