WebPBMCs were cultured in complete tissue culture medium with Phorbol 12-Myristate 13-Acetate (PMA; Sigma, 50 ng/ml) and Ionomycin (Sigma, 500 ng/ml) in the presence of BD GolgiPlug ... (Cat. No. 554723). The cells were then stained with BD Horizon™ BUV395 Mouse Anti-Human CD4 (Cat. No. 564724) and either PerCP-Cy5.5 Mouse IgG2a, κ ... http://aammt.tmmu.edu.cn/html/202412057.htm
Unique Phenotypes of Heart Resident Type 2 Innate Lymphoid Cells
WebIntracellular cytokine and chemokine detection often requires stimulation of defined cell types prior to analysis. Refer to this list below to learn how to induce commonly analyzed cytokine and chemokine expression, as well as the … Webplus ionomycin (1 μg/ml) 4-6 hours monensin or brefeldin A CD4* IL-5 TRFK Purified CD4+ T cells stimulated with immobilized anti-CD3 (10 μg/ml) + soluble anti-CD28 (2 … chinese food in largo
What the concentration of ionomycin and PMA used for …
WebBD GolgiPlug been found to have differential effects on intracellular cytokine staining that is time, activator and cytokine dependent, therefore the need to activate cells for periods … WebAnnotations: Iono=Ionomycin; PMA=Phorbol Myristate Acetate; LPS=Lipopolysaccharide; 2d=2 day culture; 5hr=5 hour culture; LPS for activation of human PBMC obtained from Sigma (#L-8274) Non-cytokine proteins: intracellular staining quick guide For Research Use Only. Not for use in diagnostic procedures. WebProcedure for Using BD GolgiStop™: Add 4 µl of BD GolgiStop™ for every 6 mL of cell culture (e.g., ~10^6 cells/mL) and mix thoroughly. Treatment of stimulated cells for 4 to 6 … grand lake of the cherokees map