High column
WebEasiest way to go about it is to remove the column (which you seem to have done already) and then you can open the purge valve on the pump. If the pressure is high with purge valve open, it means maybe your frit is the issue or something before the purge valve. WebIf the column temperature is increased, the chromatographic separation process becomes faster. A rule of thumb for reversed-phase isocratic separation predicts a retention time …
High column
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Web10 de nov. de 2024 · If high pressure is observed, the blockage is in the rotor seal within the injection valve. Replace the rotor seal. Check the Thermostatted Column Compartment / Multicolumn Thermostat: When the pressure in bypass is still high, disconnect the inlet to the column compartment / heat exchanger (Figure 1, Figure 8) and check the WebCorrecting High Back Pressure • Check pressure with/without column - many pressure problems are due to blockages elsewhere in the system. If Column pressure remains …
WebThere are many different causes to “fronting” or “tailing” peaks, but most can be easily remedied. For example, fronting peaks are often caused by column overload or overpacking. Similarly, tailing peaks can be caused by underpacking, or by having a sample that is too viscous. WebHiQ silTM HPLC Columns KYA Technologies Coporation The solution for your chromatography . KYA Technologies Corporation Japan KYA Technologies Corporation, Tokyo, Japan was established in 1998 to develop a range of high quality HPLC products. Over the last 12 years we have developed a complete range of columns in the HiQ sil …
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WebHPLC columns have an inlet and outlet frit to hold the packing inside the column. Many times, column pressure is caused from the inlet frit clogging. It is possible to attempt to backflush the HPLC column to get back to your “normal” operating pressure. How to backflush an HPLC column Reverse HPLC column direction
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