Fusion index myoblast

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August undefined, 2024

WebThe differentiation of C2C12 cells was studied over a period of seven days and was quantified; we report the differentiation parameters of (1) fusion index, (2) degree of maturation, (3) alignment, and (4) response to electrical pulse stimulation (EPS). Hybrid structures with the smallest arc degree (hybrid 30°) showed the best results for all ... WebNational Center for Biotechnology Information

Kinetin stimulates differentiation of C2C12 myoblasts - PLOS

WebMay 8, 2024 · The fusion index was calculated as the number of nuclei incorporated into myotubes expressed as a percentage of the total number of nuclei in the image frame ... Myogenin has been extensively reported to be a late marker of myoblast fusion, required for terminal differentiation (Tan et al., 2015). WebFeb 14, 2024 · ICC allows for quantification of the fusion index, which is defined as the percentage of myoblast nuclei that have fused into fibers in a single time point . Fusion indices are the main tool for assessment of the differentiation potential of myoblasts . Newer live cell imaging tools allow assessment of myoblast differentiation in real-time. installpro batibouw

Myoblast fusion: when it takes more to make one - PubMed

WebMar 16, 2024 · In addition, IGF-1 supplementation could alleviate impaired myoblast differentiation caused by mitophagy deficiency, as evidenced by increased fusion index and myosin heavy chain expression. These findings provide new insights into the role of IGF-1 signaling and suggest that IGF-1 signaling can serve as a target for the research and ... WebDec 1, 2016 · Myoblast fusion, which is essential for muscle development, regeneration, and repair, can be assessed in vitro via the calculation of a fusion index. Traditionally, … install privacy fence over chain link fence

Kinetin stimulates differentiation of C2C12 myoblasts - PLOS

SIRT3, a mitochondrial NAD⁺-dependent deacetylase, is …

WebJun 22, 2024 · The fusion index was determined to estimate the fusion capacity of the cultured myoblasts within the different stimulation conditions. The fusion index was found to be slightly increased in the 22 h per day stimulation condition (77 ± 7%) compared to statically cultured myotubes (69 ± 9%) (Figures 2A,B). WebJun 22, 2024 · The fusion index was determined to estimate the fusion capacity of the cultured myoblasts within the different stimulation conditions. The fusion index was … install printshop on windows 10WebFeb 17, 2024 · The QKI genes encode RNA-binding proteins regulating cell proliferation, differentiation, and apoptosis. The Goat QKI has six isoforms, but their roles in myogenesis are unclear. In this study, the six isoforms of the QKI gene were overexpressed in goat myoblast. Immunofluorescence, qPCR and Western blot were used to evaluate the … jim longborg pitched with which arm

"WebJun 22, 2024 · In 2D, enhanced myogenesis was observed, as evidenced by an increased myotube diameter and fusion index. Second, 2D results were translated towards 3D … " - Fusion index myoblast

Fusion index myoblast

Enhancing Myoblast Fusion and Myotube Diameter in Human 3D …

WebNov 4, 2024 · The differentiation index and fusion index were counted. Zfp422-KO myoblasts coundn't fuse to form myotube. e Zfp422 mRNA level in C2C12 cells after … WebAs far as I know calculating the fusion index with imageJ relies on image segmentation, which means the nuclei and cell membranes need to be …

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WebOct 19, 2024 · The fusion index of wild-type cultures was ∼58% after 7 days of differentiation, with an average of 6.9±2.1 nuclei per myotube (mean±s.d.; ... Taken together, our findings indicate that the regulation of myoblast fusion is more complex than previously anticipated and may involve both CDC50A-dependent (including different … WebMay 1, 2010 · Cell-cell fusion is a crucial and highly regulated event in the genesis of both form and function of many tissues. One particular type of cell fusion, myoblast fusion, is a key cellular process that shapes the formation and repair of muscle. Despite its importance for human health, the mechanisms un …

WebMyoblast fusion is essential for formations of myofibers, the basic cellular and functional units of skeletal muscles. Recent genetic studies in mice identified two long-sought … WebApr 30, 2014 · To determine the potential role of Stac3 in myoblast differentiation, C2C12 myoblasts transfected with Stac3 siRNAs or scrambled siRNA were induced to …

Webwww.ncbi.nlm.nih.gov WebDownload scientific diagram Comparison of myotube density and fusion index after C2C12 treatment. C2C12 cells were differentiated for 4 days in the presence of 10 μM prostaglandin D2 (PGD2), 10 ...

WebJun 10, 2024 · The fusion index is commonly used in muscle cell culture assays to determine the amount of myoblast fusion [6,7,8,9]. To this end, nuclei are visualized using DNA binding compounds like Hoechst, and myotubes are stained using fluorescent labelled antibodies for structural muscle protein, mainly myosin heavy chain (MyHC) among others.

WebAug 31, 2024 · Moreover, the fusion index value (0.77 ± 0.06) ... RNA analysis and fluorescence in situ hybridization. For each investigated cell population, the myoblast fusion index was calculated 27. jim long aviation flight schoolWebDec 9, 2014 · The inhibition of SIRT3 expression mediated by the stable integration of SIRT3 short inhibitory RNA (SIRT3shRNA) in C2C12 myoblasts, resulted in: 1) abrogation of terminal differentiation - as evidenced by a marked decrease in the myoblast fusion index and a significant reduction of Myogenin, MyoD, Sirtuin 1 and Troponin T protein … jim long californiaWebSep 15, 2024 · Interestingly, myoblast fusion was significantly decreased in response to co-culture with macrophages, while the effect of fibroblasts on fusion was density … jim longborg hall of fameWebDec 16, 2024 · The fusion index is indicative of the level of differentiation from myoblasts into myotubes ... Snyman C, Niesler C. Analysis and quantification of in vitro myoblast … jim long concrete mountain home arWebNov 18, 2024 · In contrast, in C2C12 cells overexpressing Nox4, the fusion index was increased, whereas the differentiation index and MHC and myogenin protein expression were not affected compared to control. Interestingly, the expression of myomaker (Tmem8c), a fusogenic protein that controls myoblast fusion, was reduced in Nox4-knockdown … install private key for certificateWebMay 26, 2016 · The differentiation index (DI) was calculated as the percentage of desmin-positive cells in relation to the total number of nuclei. In order to quantitatively assess the extent of myoblast fusion, a myogenic fusion index (MFI) was determined as the average number of myogenic nuclei present in multinucleated myotubes. jim long gone thomasWebApr 1, 2024 · (B) The fusion index of differentiated immortal myoblast cultures was determined by the percentage of the number of nuclei in myotubes (>2 myonuclei) to the total number of nuclei in desmin-positive cells (n=3 for each condition, >1000 nuclei counted per experiment). (C) Nuclear RNA aggregates of expanded-CUG or -CCUG repeats in … jim long band secrets