Fastpfu buffer
WebThe 10X FastDigest Buffer is a proprietary digestion buffer which supports 100% activity of all FastDigest restriction enzymes. DNA/RNA modifying enzymes, such as ligases, … WebConsequently, Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR …
Fastpfu buffer
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WebSep 21, 2024 · The PCR reactions were conducted using the following program: 3 min of denaturation at 95°C, 27 cycles of 30 s at 95°C, 30 s for annealing at 55°C, and 45 s for elongation at 72°C, and a final extension at 72°C for 10 min. PCR reactions were performed in a triplicate 20 μL mixture containing 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM ... WebA: In a 10 µl reaction as follows: add purified PCR product, 0.2 μl of Taq DNA Polymerase, 0.5 μl of 10 mM dNTP (or 0.5 μl of 2.5 mM dATP) and 1 μl of 10xTaq Buffer. Incubate at 72°C for 5–15 minutes. TransFast ® Taq DNA Polymerase EasyTaq ® DNA Polymerase EasyTaq ® DNA Polymerase for PAGE TransTaq ®-T DNA Polymerase
WebMar 11, 2024 · A 20-μL PCR reaction system contained 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL each of the 338F and 806R primers, 0.4 μL of FastPfu polymerase, 10 ng of template DNA, 0.2 μL of BSA, and residual ddH 2 O. WebApr 7, 2024 · Methods. Amplification, library preparation and sequencing of the bacterial 16S rRNA gene. The highly variable V3-V4 region of the 16S rRNA gene was amplified from …
WebMay 25, 2024 · The breeding systems were in a greenhouse at 27.5°C with 70% relative humidity. The larvae were fed with a mixture of wheat flour and bran every two days, and … Web侯 磊，薛 蓓，薛会英 (西藏农牧学院，西藏 林芝 860000) 土壤线虫作为土壤动物中的优势类群，是草地生态系统中的消费者，关系到草地生产力的增减[1]。
WebMar 18, 2024 · Cell lysis buffer Store it at room temperature (22°C–26°C, hereafter) for up to 6 months. CRITICAL: SDS is toxic. Wear gloves and a mask to avoid skin exposure and inhalation. TE buffer Store it at room temperature for up to 1 year. 1× B&W buffer Store it at room temperature for up to 1 year. 10 mM Tris-HCl, pH7.5
WebJan 30, 2024 · DNA samples were amplified in triplicate on an ABI GeneAmp ® 9700 PCR System (ABI, MA, USA) in PCR reactions containing 2 μl 2.5 mM dNTPs, 0.4 μl FastPfu Polymerase (Transgen Biotech, Beijing ... twitter breakfast clubWebThe amplicons were purified from 2% (w/v) agarose gels using an AxyPrep DNA gel extraction kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer's protocol. The SMRTbell libraries were prepared from the amplified DNA using blunt-ligation according to the manufacturer's protocol (Pacific Biosciences, Menlo Park, CA, USA). twitter breakfast televisionWebAug 11, 2024 · 吕燕 刘建利 李靖宇 候琳琳 孙敏 苟琪 （北方民族大学生物科学与工程学院，银川 750021） 宁夏枸杞（Lycium barbarum L.）为茄科，枸杞属，多年生落叶灌木，是典型药食同源性植物，是宁夏、甘肃、青海、内蒙古、新疆等少数民族地区脱贫的重要经济林品种，由于其极强耐旱、耐盐碱、耐贫瘠和抗沙荒 ... taking time out of your busy schedule synonymWebApr 11, 2024 · PCR reactions were performed in quadruplicate using a 20 μL mixture consisting of 4 μL of 5× TransStart FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of Forward Primer and Reverse Primer (5 μM), 0.4 μL of FastPfu Polymerase, 0.2 μL BSA, 10 ng Template DNA, and supplemented with sterile water to 20 μL. The qPCR product was … twitter breaking 911http://www.bjbalb.com/html/Nucleic-Acid-Amplification/JN0027.html twitter breaking 4 newsWebProduct Details. TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase. • Extension rate is about 2-4 kb/min. • … taking time to grieveWeb5x FastPfu FLY Buffer (with Mg2+) – GK221. $ 33.00 CAD. Format. Buy 1 x 1.2 ml 5x Fly Buffer for 33 $. Buy 5 x 1.2 ml 5x Fly Buffer for 120 $. Buy 25 x 1.2 ml 5x Fly Buffer for … taking time out meaning